My products is aromatic compound which contain 3 or more polar grp
And I have tried numerous solvents ratio like Etoac: hexane 2:1,1:2,1:1 and 1:4 but neither of them gave me satisfactory separation
Can u suggest me solvent ratio

Unfortunately, there is no general rule. Of course, if a compound is intensely colored by itself, you can often see a spot of that color on the TLC. UV-Vis spots normally look identical for similar compounds. Staining them with agents such as PMA or vanillin can lead to different shades or colors for different compounds, but after >10 years working in the lab, I can tell you that deriving general rules for this is pretty much imposible.

Proper identification on TLC has to be done by comparison with a standard (co-spot) or qualitatively by polarity.

How can I know which colour indicates to which compound? Is there any guidance or reference to that?

Cheers!

Looks like a very polar molecule with all those free -OH bonds! I’d give 25:75 hexane/EtOAc, or even pure ethyl acetate a shot. If still too polar for that, and it stays on the baseline, go with DCM/MeOH 95:5.

1) Polarity analysis (or more specifically, how exactly each molecule interacts with the stationary/mobile phase) is not that straightforward sometimes. There are cases where I’ve seen something like this that go against basic chemical intuition. Where is the alkyl chain on the alkyl resorcinol? If it’s not in one of the -OH groups, I wouldn’t be so surprised.
2) I usually observe that phenomena when something goes up too fast through the TLC. I guess it has to do with the silica gel getting saturated in amount of product that it can hold, so the shape gets uneven.